HomePage Equipment Procedures Retrieval Extraction

Lab Equipment for Adipose and Peripheral Blood Stem Cell

Collection, Culturing and Preservation

• Tulip CellFriendly Syringe Instrumentation
Standard Tissue Harvesting Cannula SuperLuerLok Adapters, $275
http://www.tulipmedical.com/canulas.html   800-325-6526

Hub: 60cc - 35cc - 10cc - Luer - SuperLuer Lok
Outside Diameters: 1.4mm (injector only) - 1.6 mm (injector only) - 2.1mm - 2.4mm - 2.7mm - 3.0mm - 3.7mm - 4.6 mm
Lengths: 8 cm (injector only) - 15cm - 25cm - 35cm

• Ultrasonic aspirators: $3500 to $4000.
http://www.tulipmedical.com/canulas.html  800-325-6526

• Byron, Coleman cannulas 800.777.3434, Concord series disposable stainless cannulas for liposuction, adipose - fat retrieval; Lysonix 3000 ultrasonic aspirator

• Collection of whole blood, (approx. 100 ml.):  unresearched
Painless blood collection system using 700-1200 nm near IR vein detection with 850 nm LED array; (Axiomatec, Japan Science and Technology, Biomaterials Center National Institute for Materials Science).

• Centrifuge, RPM to G, for red cell separation, pellet; (modified blender), select volume and RPM range.

• Containers, plastic 1 to 2 ml for cryogenic storage versus glass.

• G-CSF, Filgrastim, Neupogen= $1862 per 10 vials drugstore.com for injections, Melphlan, AMD3100, Neulasta 6 mg Amgen increase stem cells in blood by 12 times, $2755 per 1 syringe of 6 mg drugstore.com.  Stimulate white cell production: Astragalus membranaceus; 3 days on & 3 days off Echinacea purpurea Intl J of Immunopharmacology 1991 12(7) 931 to 941; Growth Hormones; L-Cystine, Tyrosine, Histidine; Cytokines: IL-3, IL-7, G-CSF, M-CSF, GM-CSF;  Prostaglandin E1 (PGE-1) stimulates myocardial angiogenesis in human chronic ischemic myocardium, induces angiogenesis and increases VEGF levels and acts as a vasodilator also stimulates CD34;  Hematopoietic Stem Cell-Stimulating Substances From Kampo (Japanese Herbal) TJ-48 2.5 grams per day;  Spirulina 2 to 3 grams;  Zinc,  Oleic (.5 mcg/ml = 2 times increase) & Linolenic Acid (about same);

• EasySep™ Human CD34 Positive Selection Cocktail:

• EasySep Magnet #18000 $875 included in kit, (up to 5 x 108 CD34+ cells may be processed per separation),  800.667.0322 Lynn, StemCell Technologies, first centrifuge and remove or pelletize red blood cells, 100 ml whole blood = 10,000 stem cells at .02% of total cell count, with 96% purity. Media has 2-year shelf life.  Contains magnetic tags for 50 billion stem cells per 1 ml.  Magnetic tag does not interfere with cell culturing.  How many tags attach per stem cell?

Chalmers Magnetic Stem Cell Sorting 703.528.2430 Frank, (Whitaker Foundation).

• Cryogenic freezer: (dry freezer storage substantially reduces sample contamination over liquid nitrogen storage, particularly when opening cold vials draws in contaminants; unless decontamination procedures are implemented).  Revco Ultima 2, ULT-7150-9, -150°C, list price: $20,216.  uses: 20 amps at 208 volts, Asheville North Carolina 800.879.7767; see: Fisher Scientific 800.766.7000; VWR: 800.932.5000

• Liquid Nitrogen Refrigerator: Taylor-Wharton (previously: Union Carbide) 34XT, 34 liter, evaporation rate 1/10 liter per day, $1033. list, 3 year warranty (without canisters, no canes supplied) 800.898.2657 Alabama (Vacuum repair cost more than new unit, lifespan of insulation uncertain).

• LN Supplies: Air Gas Puritan Medical, San Diego 800.234.5456;  Air Gas West, San Diego: 858.495.4811 ($832. for 34XT, Brody 495.2377);  Gilmore Gas: 626.443.1361;  Praxair, Long Beach: 562.437.0382;  Cryogenic Services, Long Beach: 562.439.1653;  Air Source, Long Beach: 562.426.4017;

• Trehalose cryoprotectant = 94% stem cell survival, DMSO alone = 42% survival, catalase + trehalose + DMSO better than one, membrane stabilizers and bioantioxidants (India); Cryopreservation of Stem Cells Using Trehalose: Evaluation of the Method Using a Human Hematopoietic Cell Line,  Sandhya S. Buchanan, Sherilyn A. Gross, Jason P. Acker, Mehmet Toner, John F. Carpenter, David W. Pyatt, Stem Cells and Development, June 2004, Vol. 13, No. 3: Pages 295-305; 

Ferro Pfanstiehl Laboratories, 1219 Glen Rock Ave.  Waukegan, IL 60085
Tel: (847) 623-0370,  Toll Free: (800) 383-0126, pfanstiehl-CustServ@ferro.com
www.pfanstiehl.com, Trehalose, 1 kilo minimum at: $441.

• Culture medium: Stemline™ hematopoietic Stem Cell Expansion Medium S0192, 500 ml, $114. sigmaaldrich.com  800.325.5832, 800.325.3010 order, (Provides for a 400 times increase in cell count in a 2 stage process 7 days + 7 days, 10000 cells * 400 = 4,000,000 * 400 =  1,600,000,000 (4 stages).

• Culture flasks: Corning® 25cm² Rectangular Canted Neck Cell Culture Flask with Vent Cap (#430639).

OptiCell Chambers:  Impact-resistant polystyrene frame measures 127 x 85 x 5.8 mm thick (5” x 3.35” x 0.228”). The film surfaces measure 74.8 x 65 x 0.075 mm thick (2.94” x 2.56 “ x 0.002”). Gamma sterilized, non-pyrogenic (not autoclavable).  From USA Scientific

• Electrophoresis: verification and contamination detection: (unresearched) Polymerase Chain Reaction, restriction enzyme digestion & 2D agarose gel electrophoresis; with scanning for image archiving & computer analysis.  Description of: The ElectrophoretIQ³ electrophoresis platform provides outstanding flexibility for 2D polyacrylamide gel electrophoresis. A unique 3-in-1 hybrid of first dimensional IEF separation, second dimensional SDS-PAGE and compact blotting cassettes.  Proteome Systems Inc. 14 Gill Street Woburn, Massachusetts 01801 USA Tel: +1-781-932-9477, Toll free: 866-779-7836

• Inverted microscope with fluorescent illumination, incubator, Varel (Hoffman) optics and camera port.  Zeiss Axiovert 200 with XL-3 Incubator, heated stage and micromanipulators.

• Piezo injection (minimization of cell damage): The piezo translator PM 20 is especially suitable for intracellular injection. Once the capillary is positioned at the cell, penetration, injection, and withdrawel are executed by pushing the start button.  The PM 20 is conceived primarily for utilization with our micromanipulators MMJ or DC 3K. Their standard tool holders easily accept the PM 20.  See:  http://www.marzhauser.com/engl/i_pm20.html

The micromanipulator DC 3K is made for motorized or manual movement in all three axes (X-, Y-, and Z-axis) which makes it a valuable tool for numerous intracellular applications. The manual part of the DC 3K is equivalent to the MM 33 with additional Z-axis-stop. The motorized action ensues by way of three fine positioning slides with built-in DC motors and micrometer spindle.  See: http://www.marzhauser.com/engl/i_dc.html

HomePage Equipment Procedures Retrieval Extraction

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